Fig. 2: DSB repair pathways beyond NHEJ are dispensable for generating complex rearrangements from micronuclei.

a Left: distribution of Y chromosome rearrangement types as determined by metaphase FISH following continuous passage in DOX/IAA and G418 for ~30 days. Data are pooled from two independent experiments. Right: plots depict the mean fold change in each rearrangement type as compared to WT cells. Sample sizes indicate the number of rearranged Y chromosomes examined; data are pooled from two or three individual KO clones per gene. See also Supplementary Fig. 3. b Proportion of Y chromosomes exhibiting simple or complex rearrangements, as determined by metaphase FISH, following sustained centromere inactivation. Data represent the mean of n = 2 independent experiments for WT, n = 2 KO clones for LIG4 and RAD52, and n = 3 KO clones for DNA-PKcs, XLF, 53BP1, Polθ, RAD54, and NBS1. c Cytogenetic characterization of POLQ KO sub-clones harboring complex Y chromosome rearrangements following sustained centromere inactivation. Scale bar, 10 µm. d Whole-genome sequencing analyses of Polθ KO sub-clones with complex Y chromosome rearrangements exhibiting oscillating DNA copy-number patterns. For each subclone, sequencing depth (gray dots), copy-number information (black lines; top), and inter-mutational distances (bottom) for the mappable regions of the Y chromosome are shown. Source data are provided as a Source Data file.