Fig. 5: Metagenomic information and mutations harbored by the Wolbachia genomes.
a Blobplot showing the read depth, GC content, and taxonomic origin of assembled contigs, with blob size proportional to the corresponding contig length. Contigs with an unknown taxonomic origin (green blobs) were identified as Y-linked contigs or VNTRs (Supplementary Data 7, “Methods”). b Circos plot illustrating the alignment of wMel, wISO1-1/2, and the previous assembly9, marking three mutations and nine ltrAs in wISO1-1/2. Five contigs were assembled in the ref. 9. c Deletion in GQX67_01850. Three amino acids (VSA) are removed in wISO1-1/2. d Point mutations in ltrA_2 and ltrA_7. The left-side phylogenetic tree of the ltrA gene family, based on wMel DNA sequences, shows bootstrap values around internal nodes. The scale bar represents 10% nucleotide divergence. The substantial divergence of 57.0% between the two groups necessitates the representation of this branch as a dashed line. “ψ” denotes a pseudogene with at least one premature termination codon (PTC). e Schematic representation of read alignment at the ltrA_7 locus, with the D- > N mutation marked in red. The 1548-bp genic region together with 618-bp and 135-bp flanking regions of ltrA_7 are identical to those of the other four paralogous genes. A few Illustrative reads spanning the mutation are shown. Long reads align to this locus together with upstream or downstream regions, while short reads cannot differentiate this locus from paralogous loci.
