Fig. 1: Design of Nδ-vinyl histidine for enzyme evolution.
From: Genetically encoded Nδ-vinyl histidine for the evolution of enzyme catalytic center
A A cartoon representation of the His in the catalytic center of esterase- and heme-dependent enzymes was generated using PyMOL (the structures of esterase and myoglobin were generated from the PDB files 6q7o and 5oj9). B pKa comparison of imidazole and its derivatives. C Comparison of the surface charges of imidazole and its derivatives. D pKa ranges of natural amino acid sidechains and imidazole derivatives. E Scheme of the evolution of enzymes enabled by Nδ-vinyl histidine. Source data are provided as a Source Data file.
