Fig. 4: The incorporation of δVin-H improved the activity of OE1.3 under acidic conditions.
From: Genetically encoded Nδ-vinyl histidine for the evolution of enzyme catalytic center
A The structure of OE1.3-δVin-H was regenerated from the pdb file 6q7o. B, C Expression and purification of OE1.3-δMeH/δVin-H and LCMS validation of OE1.3-δVin-H. The data of Fig. 4B is the representative data from similar results after three independent experiments. D–F Comparison of the activities of OE1.3-His, δMeH, and δVin-H at pH = 5.5. The data in E, F are presented as mean values ± SEM (n = 3, independent experiments). Source data are provided as a Source Data file.
