Fig. 6: Evolution of myoglobin via the site-specific incorporation of δVin-H.
From: Genetically encoded Nδ-vinyl histidine for the evolution of enzyme catalytic center
A The structure of myoglobin-δVin-H regenerated from pdb file 5oj9 (illustrative purposes only and does not represent the actual structure). B The cycloaddition reactivity of myoglobin is improved by replacing the axial coordinate His with δVin-H. C–E The improvement in the activity of myoglobin by δVin-H was demonstrated in both electron-deficient and electron-rich styrene substrates. The yields in C–E are presented as mean values ± SD (n = 3, separate experiments) and the liquid chromatogram spectrum is a representative result of these three replicates. Source data are provided as a Source Data file.
