Fig. 5: rs781745126-T carriers have less LAG3 mRNA expression in stimulated T-cell subsets. | Nature Communications

Fig. 5: rs781745126-T carriers have less LAG3 mRNA expression in stimulated T-cell subsets.

From: Start codon variant in LAG3 is associated with decreased LAG-3 expression and increased risk of autoimmune thyroid disease

Fig. 5

LAG3 expression was assessed in data from single-cell RNA (scRNA) sequencing (in-house data) of unstimulated (red) and stimulated (blue) peripheral mononuclear cells (PBMCs, 24-h stimulation with anti-CD3/anti-CD28) from 26 heterozygous carriers (CT) and 766 non-carriers (CC) of rs781745126-T. The y-axis shows the expression in TPM (Transcripts Per Million) and the distribution is shown by box-plots (outliers, 10–90th percentile, interquartile range, median levels). LAG3 expression was increased after stimulation, but this upregulation was lower among carriers of rs781745126-T than among non-carriers on CD8+ T-cells (43% lower, P = 1.06 × 10−5), CD4+ memory T-cells (35% lower, P = 8.59 × 10−4) and naïve T-cells (45% lower, P = 3.36 × 10−6). For all subsets and information about marker genes and cell type classification of scRNA-sequencing data, see Supplementary Data 12, Supplementary Data 13, Supplementary Information and “Methods” section. Two-sided P-values were computed using Satterthwaite’s method for mixed-effect models.

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