Fig. 1: Dietary TMB self-administration stabilizes the retinal transcriptome and mitigates the disease phenotype in rd10 mice.

A Study design. B Representative examples of drug doses via dietary tamsulosin, metoprolol, and bromocriptine (TMB) coadministration, obtained from the blood and the target tissues at 12:00 a.m. The blood level of bromocriptine did not reach the sensitivity limit of the assay, so it is labeled as “not detected” (n.d.). C Representative optical coherence tomography (OCT) images. The plus sign (+) indicates outer nuclear layer (ONL). ONH, optic nerve head. D ONL thickness and retinal detachment, as measured from OCT images. E, F Group-averaged electroretinogram (ERG) waveforms in response to green E and UV F stimuli under photopic conditions in a representative cohort. G, H M-cone- G and S(UV)-cone-dominant H ERG b-wave amplitudes. I Venn diagrams of upregulated and downregulated genes in retinal bulk RNA-sequencing. J, K Transcriptome heatmap of 5000 most-abundant nuclear genes J, and 10 most-abundant mitochondrial-encoded genes K, that show an expression change compared to WT in dark-reared (DR) rd10 mice (at P28, non-treated), or experimental rd10 mice (P37, changed from dark to vivarium/cyclic light-rearing [CLR] at P29) that were treated with vehicle (veh) or TMB. The asterisks signify statistical differences (adjusted P < 0.05) compared to WT expression level. L Heatmap of regulation in a set of genes associated with retinitis pigmentosa (RP). M Heatmap of regulation in genes encoding adrenergic and dopamine receptors, and major catecholamine synthetizing or degrading enzymes. The statistical analysis employed for graphs in D was the Mann–Whitney U-test (two-tailed), and for graphs G and H the Kruskal–Wallis test was followed by Dunn´s multiple comparisons test; The asterisks signify: ***P < 0.001, ****P < 0.0001. Bar graph data are presented as mean ± SD. Source data are provided as a Source Data file.