Fig. 5: Monotherapies with tamsulosin, metoprolol, or bromocriptine are not effective in delaying retinal degeneration in rd10 mice.

Rd10 mice were dark-reared between P0-P28, and treatments were started at P28. One day later (P29), mice were transferred to normal laboratory housing conditions, and treatment effect was tested at P36. The drug effect was evaluated using four different parameters: photopic M- or S(UV)-cone targeted ERG b-wave amplitudes, ONL thickness, or retinal detachment from the RPE. A–D Tamsulosin (T) monotherapy data. E–H Metoprolol (M) monotherapy data. I–L Bromocriptine (B) monotherapy data. M–P Tamsulosin/bromocriptine (TB) dual-treatment data contrasted with TMB triple-treatment. Q–T Tamsulosin and metoprolol (TM) dual-treatment data contrasted with TMB triple-treatment. U–X Metoprolol and bromocriptine (MB) dual-treatment data contrasted with TMB triple-treatment. The ONL thickness and retinal detachment data were statistically analyzed by the non-parametric Mann–Whitney U-test (two comparisons; two-tailed) or the Kruskal-Wallis test (three comparisons) followed by Dunn´s multiple comparisons tests. All ERG data were analyzed using repeated measures two-way ANOVA with Geisser-Greenhouse correction and followed by Bonferroni post hoc tests. Asterisks illustrate significant Mann-Whitney or Bonferroni test results: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. The pound signs illustrate significant between-subjects ANOVA main effects: ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, ^####P < 0.0001. Data are presented as mean ± SD. Source data are provided as a Source Data file.