Fig. 4: miR-1204 promotes senescence-associated secretory phenotype (SASP) component accumulation and contractile phenotype loss in vascular smooth muscle cells (VSMCs).
From: Aging aggravates aortic aneurysm and dissection via miR-1204-MYLK signaling axis in mice
A Representative images of senescence-associated-β-galactosidase (SA-β-gal) staining (upper panel) and quantification (lower panel) in VSMCs transfected with miR-1204 mimics or miR-control (miR-ctl). n = 6 biological replicates. Statistical analysis was performed using two-tailed Student’s t test. Scale bar, 100 μm. B Quantification of mRNA levels of SASP components in VSMCs transfected with miR-1204 mimics or miR-ctl. n = 6 biological replicates. Statistical analysis was performed using two-tailed Student’s t test. C Interleukin 6 (IL-6) concentration in the culture supernatant of VSMCs transfected with miR-1204 mimics or miR-ctl combined with angiotensin II (AngII) treatment. n = 6 biological replicates. Statistical analysis was performed using a one-way ANOVA followed by Tukey’s multiple comparison test. D Monocyte chemotactic protein 1 (MCP-1) concentration in the culture supernatant of VSMCs transfected with miR-1204 mimics or miR-ctl combined with AngII treatment. n = 6 biological replicates. Statistical analysis was performed using a one-way ANOVA followed by Tukey’s multiple comparison test. E–G Representative plots (upper) and quantification (lower) of immunoblot analysis of contractile markers, α-smooth muscle actin (α-SMA), smooth muscle protein 22 (SM22), and myosin heavy chain 11 (MYH11) in VSMCs transfected with miR-1204 mimics or miR-ctl combined with AngII treatment. n = 6 biological replicates. Statistical analysis was performed using a one-way ANOVA followed by Tukey’s multiple comparison test. H Representative images (left) and quantification (right) of immunofluorescence staining of CD68, MYH11, α-SMA, and SM22 in mouse aortas in the indicated groups. n = 17 biological replicates for miR-1204 + AngII group. n = 19 biological replicates for other groups. Statistical analysis was performed using a one-way ANOVA followed by Tukey’s multiple comparison test for α-SMA and SM22 quantification, by two-tailed Welch’s ANOVA followed by Dunn’s multiple comparisons for CD68 and MYH11 quantification. Scale bar, 100 μm. Data are presented as mean ± SD. Source data are provided as a source data file.
