Fig. 4: Proteome analysis of M. bovis BCG Danish 1331 WT and ∆lamH.
a Volcano Plot of proteome alterations observed between M. bovis BCG and ΔlamH visualised as log2(WT/ΔlamH) and -log10(P values). Proteins of interest in the LAM biosynthetic pathway are denoted in red. Plots showing quantitation of statistically significant proteins are found in Supplementary Fig. 5. Significance was determined using two-tailed unpaired t tests, and multiple hypothesis correction was undertaken using a permutation-based FDR approach. The complete list of differentially expressed proteins is in Supplementary Data 1. b Eight proteins associated with LAM biogenesis were observed in the proteomics dataset. These have been annotated next to the bond on the simplified LAM structure for which they are understood to generate. Proteins coloured red meet statistical significance for being lower in abundance (Supplementary Fig. 5). Those in orange appear lower in abundance but do not meet statistical significance. Those in black have no change associated with their expression. Source data are provided as a Source Data file.
