Fig. 5: LamH-catalysed AM turnover is required for efficient exit from lag phase. | Nature Communications

Fig. 5: LamH-catalysed AM turnover is required for efficient exit from lag phase.

From: The mycobacterial glycoside hydrolase LamH enables capsular arabinomannan release and stimulates growth

Fig. 5

a Growth kinetics of the indicated strains. Complemented—∆lamH L5::pMV306-lamH, Catalytic null—∆lamH L5::pMV306-lamHD96A. b The ∆lamH strain was grown either in spent media from wild-type bacteria, fresh media or media supplemented with 0.5 mg/mL AM. The values for WT are included from (a) for comparison. WT— M. bovis BCG Danish 1331, Mutant—M. bovis BCG Danish 1331 lamH::Himar1, Complement—M. bovis BCG Danish 1331 lamH::Himar1, L5::pMV306-lamH, Catalytic Null—M. bovis BCG Danish 1331 lamH::Himar1, L5::pMV306-lamHD96A. For all growth curves the shaded area represents the 95% confidence intervals of three biological replicates with the centre of measure defined as the mean and n = 3 biological replicates. Source data are provided as a Source Data file.

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