Fig. 6: Reduction of lamH expression leads to a decrease in fitness in macrophages.

a The parental M. tuberculosis H37Rv strain, including pLJR965 at the L5 site, was used to infect THP-1-derived macrophages in the presence and absence of ATc. Bacterial intracellular viability was examined at the indicated time points. b A strain carrying pLJR965 with a lamH guide RNA at the L5 site was also used to infect THP-1-derived macrophages similarly. This strain manifested a growth defect in the presence of ATc that was not observed when ATc was omitted from the culture medium. Data presented are mean and standard error and are representative of two independent experiments each with three biological replicates with similar results. Significant differences were calculated by a multiple t test using a two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli, with Q = 1%. Each row was analysed individually without assuming a consistent standard deviation. Number of tests performed: 4. ****, 24 h P = 0.00079; 48 h P = 0.00007; 72 h P = 0.00024. Source data are provided as a Source Data file.