Fig. 2: Characterization of aptamer-displaying, flexible M13 nanofibers on magnetic beads (A-f-M13-MB). | Nature Communications

Fig. 2: Characterization of aptamer-displaying, flexible M13 nanofibers on magnetic beads (A-f-M13-MB).

From: Harnessing virus flexibility to selectively capture and profile rare circulating target cells for precise cancer subtyping

Fig. 2

a The cartoon illustration indicated the specific interaction between Ni-IDA on MBs and 6His tag on pIII of phage. Cartoon shown in Fig. 2a created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en). TEM images revealed that M13 nanofibers were anchored on MBs in an end-on manner. M13 nanofibers are indicated by arrows. b The much higher binding efficiency of 6His-M13 on Ni-IDA MBs compared to wild type (WT) M13 indicated the successful construction of 6His-M13 (n = 3 samples, mean ± s.d.). The Q-TOF LC/MS spectra of WT-M13 phage (c) and N3-M13 phage (d). The peak at 5238 m/z confirmed the presence of pVIII in WT-M13, while the peak difference between 5238 m/z and 5585 m/z fitted the molecular weight of N3-PEG, indicating presence of N3-PEG. e Fluorescence microscopic images of FAM-A-f-M13-MB (upper) and control (lower). Aptamer was labeled with FAM, thus those MBs anchored with A-f-M13 emitted green fluorescence whereas the control MBs that anchored with WT-M13 didn’t. Scale bar: 20 μm. Source data are provided as a Source Data file. WT-M13: wild type M13 phage; 6His-M13: M13 with 6His tag displayed on pIII; N3-M13: 6His-M13 with N3-PEG decorated on pVIII; Apt-M13: N3-M13 with aptamer “clicked” onto pVIII.

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