Fig. 1: Aspergillus melanin inhibits transepithelial migration of neutrophils.
From: Fungal melanin suppresses airway epithelial chemokine secretion through blockade of calcium fluxing
a Schematic of neutrophil migration assay. Neutrophil migration across primary human airway epithelial cells (hAECs) (b) or H292 cells (c) measured by apical MPO assay following infection (2 h hAECs; 1 h H292) with 1 × 107/cm2 wildtype B5233 or ΔpksP A. fumigatus conidia in the presence or absence of purified A. fumigatus melanin ghosts (MG; 5 × 107/cm2). Negative control of media (HBSS) or MG alone was included. Data represents the number of neutrophils as determined by the standard curve. Data are represented as mean ± SEM. N = 4 (primary hAECs controls [HBSS, MG]); N = 6 (primary hAECS infections and all H292 cells) from three experiments; One-Way ANOVA with Tukey’s multiple comparisons test; **p = 0.006, ***p = 0.0005, ****p < 0.0001. Source data are provided as a Source Data file.
