Fig. 5: Melanin abolished the transepithelial gradient of CXCL8 required for neutrophil transmigration, with a durable impact on CXCL8 secretion.
From: Fungal melanin suppresses airway epithelial chemokine secretion through blockade of calcium fluxing
a CXCL8 expression in the media from the apical (A) and basolateral (BL) sides of H292 cells was measured by ELISA. H292 cells were infected for 6 h with P. aeruginosa PAO1 strain (PSA) and Aspergillus melanin ghosts (MG; 5 × 107/cm2) together or alone. Media alone (HBSS) was used as a negative control. n = 6 (HBSS, PSA, MG BL) or n = 5 (PSA + MG, MG A [due to technical error]) from three replicates; two-way ANOVA with Sidak’s multiple comparisons test. ****p < 0.0001. (b) An ELISA assessed apical CXCL8 levels following a 24 h stimulation with PSA or TNFα (100 ng/mL) in the absence or presence of MG (5 × 107/cm2). n = 3 from three replicates; one-way ANOVA with Tukey’s multiple comparison test. Data are represented as mean ± SEM. ***p = 0.0011, ****p = 0.0001. Source data are provided as a Source Data file.
