Fig. 7: Epithelial calcium fluxing dampened by fungal melanin.
From: Fungal melanin suppresses airway epithelial chemokine secretion through blockade of calcium fluxing
a H292 cells were stimulated with media (HBSS) alone or melanin ghosts for 30 min followed by a 1 h incubation with ionomycin (2 µM) and calcium chloride (1.3 mM) for 2 h. Calcium fluxing measured by plate reader every 15 sec. Cell stimulated with media in the presence or absence of melanin ghosts produced virtually identical values. Data represent three technical replicates. b Representative DIC (top panel) and confocal (bottom panel) images of H292 calcium fluxing by fluo-4 in the presence or absence of melanin ghosts when treated with media alone (HBSS without calcium or magnesium) or ionomycin and calcium. Images from 10 min timepoint and representation of one region of interest in one set of experiments. Scale bar = 10 µm. Source data are provided as a Source Data file.
