Fig. 8: Melanin disrupts actin structure in airway epithelial cells.
From: Fungal melanin suppresses airway epithelial chemokine secretion through blockade of calcium fluxing
a Representative images of phalloidin (green) and DAPI (blue) stained H292 cells stimulated with media alone (HBSS), P. aeruginosa PAO1 strain (PSA; 1 × 107/cm2) ± MG, or MG alone (5 × 107/cm2) for 6 h. Images are a representation of one region of interest in one set of experiments Scale bar = 10 µm. b Mean fluorescence intensity (MFI) over area for phalloidin staining images (a). n = 3 regions of interest (PSA only) or n = 4 (HBSS, PSA + MG, MG). One-way ANOVA with Tukey’s multiple comparisons test; *p < 0.05 compared to HBSS (PSA + MG [p = 0.0337]; MG [p = 0.0231]). c Representative of F actin (top blot), G actin (1:20 dilution; middle blot), and total actin (bottom blot) measured by Western blot from three independent experiments. H292 cells were stimulated with medial alone (HBSS), P. aeruginosa PAO1 strain (PSA; 1 × 107/cm2) ± MG, or MG alone (5×107/cm2), DMSO vehicle control, or latrunculin A (LatA) for 6 h. Phalloidin was additionally used as a positive control. (d) Quantification of three biological replicates F/G actin ratio from (b). N = 3; Two-way ANOVA with Tukey’s multiple comparisons test; *p = 0.0031, **p = 0.0025. Source data are provided as a Source Data file.
