Fig. 3: BAP2 is necessary to overcome chronic ER stress, and the Na-1 allele, but not the Est-0 allele, complements bap2 ER stress sensitivity.
a Representative images of Col-0, bap2, ire1a ire1b and the transgenic lines expressing Na-1 (pBAP2Na-1:BAP2) or Est-0 (pBAP2Est-0:BAP2N67S) genomic fragment (promoter and coding region) in the bap2 background germinated on media containing the indicated concentrations of TM or DMSO grown for 10 days. b Relative shoot fresh weight of Col-0 (green dots), bap2 (blue triangles), and ire1a ire1b (purple squares) seedlings treated as indicated in (a) Data represent means ± SEM among biological replicates (n = 18). Statistical significance was determined using a factorial linear mixed model framework followed by post-hoc testing using two-sided Tukey’s HSD test (multiple testing-controlled thresholds used was P < 0.05). c Diagram of Col-0, Na-1, and Est-0 BAP2 protein sequence. Mutation in the Est-0 sequence is indicated in red. The C2 domain is indicated in dark gray. d, e Relative shoot fresh weight of pBAP2Na-1:BAP2 (d) or pBAP2Est-0:BAP2N67S transgenic lines treated as indicated in (a) (Col-0 values are represented in green dots, bap2 values in blue triangles, independent transgenic line #1 values in purple squares, and independent transgenic line #2 values in orange rhombuses). Data represent means ± SEM among biological replicates (n = 18). Statistical significance was determined using a factorial linear mixed model framework followed by post-hoc testing using two-sided Tukey’s HSD test (multiple testing-controlled thresholds used was P < 0.05). Source data are provided as a Source Data file.
