Fig. 4: The BAP2 Est-0 allele contributes to Est-0 ER stress sensitivity while the Na−1 allele partially attenuates it.

a Representative images of Col-0, Est-0, and transgenic lines expressing Na-1 (pBAP2Na-1:BAP2) or Est-0 (pBAP2Est-0:BAP2N67S) genomic fragment (promoter and coding region) in Est-0 background seedlings germinated on media containing the indicated concentrations of TM or DMSO grown for 10 days. (b, c) Relative shoot fresh weight of pBAP2Na-1:BAP2 (b) or pBAP2Est-0:BAP2N67S (c) transgenic lines treated as indicated in (a) (Col-0 values are represented in green dots, bap2 values in blue triangles, independent transgenic line #1 values in purple squares, and independent transgenic line #2 values in orange rhombuses). Data represent means ± SEM among biological replicates (n = 18) for relative shoot weight. Statistical significance was determined using a factorial linear mixed model framework followed by post-hoc testing using two-sided Tukey’s HSD test (multiple testing-controlled thresholds used was P < 0.05). Source data are provided as a Source Data file.