Fig. 7: BAP2 is a pro-death effector when IRE1 is dysfunctional. | Nature Communications

Fig. 7: BAP2 is a pro-death effector when IRE1 is dysfunctional.

From: Programmed cell death regulator BAP2 is required for IRE1-mediated unfolded protein response in Arabidopsis

Fig. 7

a Representative images of Col-0, bap2, ire1a ire1b, and ire1a ire1b bap2 seedlings germinated on media containing the indicated concentrations of TM or DMSO for 10 days. b Relative shoot fresh weight of seedlings treated as indicated in (a). c Quantification of the relative percent electrolyte leakage to determine the extent of cell death in Col-0, bap2, ire1a ire1b, and ire1a ire1b bap2 seedlings treated as indicated in (a). d, e Quantification of relative percent of accumulation of H2O2 by an Amplex Ultra Red assay (d) and the relative percent electrolyte leakage (e) in Col-0, bap2, ire1a ire1b, and ire1a ire1b bap2 seedlings treated with 1 μg mL−1 TM or DMSO for 48 hours. b–e Data represent mean ± SEM among biological replicates (n = 28 for (b), n = 12 for (c), n = 9 for (d) and (e). Statistical significance was determined using a factorial linear mixed model framework followed by post-hoc testing using two-sided Tukey’s HSD test (multiple testing-controlled threshold used was P < 0.05). Color legend: Col-0 in green dots, bap2 in blue triangles, ire1a ire1b in purple squares, and ire1a ire1b bap2 in orange rhombuses. f, g qRT-PCR analysis of NAC089 in Col-0 (green dots), bap2 (blue triangles), ire1a ire1b (purple squares), and ire1a ire1b bap2 (orange rhombuses) seedlings after 3- h or 6- h pulse treatment with 0.5 μg mL−1 TM or DMSO (f) or in Col-0 (green dots), bap2 (blue triangles), bzip28 (purple squares), bzip60 (orange rhombuses), bzip28 bap2 (brown invert triangles) and bzip60 bap2 (violet hexagons) seedlings after 6 h pulse treatment with 0.5 μg mL−1 TM or DMSO (g) or Col-0 (green dots), bap2 (blue triangles), bzip28 bzip60 (purple squares) and bzip28 bzip60 bap2 (orange rhombuses) seedlings after 6 h pulse treatment with 0.5 μg mL−1 TM or DMSO (h). Values are presented relative to the indicated DMSO control. Transcription of UBQ10 was used as an internal control. Data represent mean ± SEM among biological replicates (n = 12 for (f), n = 8 for (g) and (h). Statistical significance was determined by Student’s unpaired two-tailed t-test (P-value is shown; ns, not significant). Source data are provided as a Source Data file.

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