Fig. 2: Knockout of Nrp1 in TECs reduces I-R-induced kidney damage and fibrosis.

A Levels of Nrp1 measured by RT-qPCR (For Sham, n = 7; Vehi+IRD5, n = 6; Tmx+IRD5, n = 7). B A schematic diagram illustrating the experimental scheme of I-R analysis. C Ratio of kidney weight versus body weight of mice. Plasma blood urea nitrogen (BUN) concentrations and creatinine (CR) concentrations in sham, Vehi + IR, or Tmx + IR at 5 days (For Sham, n = 8; Vehi+IRD5, n = 7; Tmx+IRD5, n = 7) and 14 days (n = 7 per group). D Representative micrographs and corresponding statistical scores of periodic acid-Schiff (PAS) (For Sham, n = 8; Vehi+IRD5, n = 7; Tmx+IRD5, n = 7), Kim1 immunofluorescence staining (For Sham, n = 8; Vehi+IRD5, n = 7; Tmx+IRD5, n = 7) and plasma Kim1 detected by enzyme linked immunosorbent assay (ELISA) (For Sham, n = 5; Vehi+IRD5, n = 6; Tmx+IRD5, n = 6) on day 5 after IR in mice. The assessment of renal tubular damage involves evaluating tubular necrosis, cast formation, tubular dilation, and brush border loss. Scores are assigned to indicate the degree of damage: 0 for no damage, 1 for 10% damage, 2 for 11–25% damage, 3 for 26–45% damage, 4 for 46–75% damage, and 5 for more than 76% damage. E Representative micrographs and corresponding statistical scores of PAS, Masson, Sirius red, Kim1 immunofluorescence staining and plasma Kim1 detected by ELISA on day 14 after IR in mice (n = 7 per group). F Expression levels of kidney damage indicators (Havcr1 and Lcn2) and fibrosis-related factors (Acta2, Pdgfrb, Col1a1 and Fn1) at day 14 after IR determined using RT-qPCR (n = 5 per group). *P < 0.05, **P < 0.01, ***P < 0.001 as determined by one-way ANOVA. Scale bar, 20 μm. Data represent mean ± SEM. Source data are provided as a Source Data file.