Fig. 3: Fitness data from complementation assays. | Nature Communications

Fig. 3: Fitness data from complementation assays.

From: Barcoded overexpression screens in gut Bacteroidales identify genes with roles in carbon utilization and stress resistance

Fig. 3

The average fitness score from both replicates is plotted for each fragment. Fragments that cover the complete gene are indicated in green. a BT2158 and BT4448 in B. theta, and a homolog (BACUNI_RS05440), were beneficial for the ΔBT2158 mutant during growth in leucrose and palatinose. b Growth curves of B. theta ΔtdkΔBT2158 complemented with either BT2158 or a homologous hit. Complemented isolates, the Δtdk parental strain, and the ΔtdkΔBT2158 mutant were cultured in the VB medium with 20 mM trehalose. Strains 1–2 encode BT2158, strains 3–4 encode BT4448, and strains 5–6 encode BACUNI_RS05440. See Supplementary Data 7 for the precise genomic boundaries of cloned fragments. Each strain was grown in replicates of 4. OD600 values are pathlength-corrected and blank-normalized. c CdsB was enriched in the ΔtdkΔBT3703 mutant in ɑ-cyclodextrin. d RB-TnSeq fitness scores for CdsB (BT2080/BT_RS10540) are averages across all 37 CdsB transposon mutants in the B. theta library and are log2 fold-changes that were previously reported (x-axis)11. Scores are highlighted in red for assays in the VB minimal medium and in black for the BHIS rich medium. The y-axis is random scatter for visualization.

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