Fig. 6: Genes involved in lipid biosynthesis, membrane protein translocation, and capsular polysaccharide biosynthesis increase tolerance to ceftriaxone, taurocholate, or cholate. | Nature Communications

Fig. 6: Genes involved in lipid biosynthesis, membrane protein translocation, and capsular polysaccharide biosynthesis increase tolerance to ceftriaxone, taurocholate, or cholate.

From: Barcoded overexpression screens in gut Bacteroidales identify genes with roles in carbon utilization and stress resistance

Fig. 6

Fragment plots for (a) fadD (HMPREF1079_RS19625) in 0.5 mM ceftriaxone, (c) phosphoethanolamine biosynthesis genes (HMPREF1079_RS14395, HMPREF1079_RS14390) in 10 mM taurocholate, and (e) yajC (BACUNI_RS18600) in 10 mM taurocholate. Growth curves of individual hits expressed in B. theta: (b) 2-ketoisovalerate oxidoreductase (HMPREF1077_RS19335, _RS17900, _RS17905, _RS17910) and fabX (HMPREF1077_RS10180) in 1 mM ceftriaxone; (d, f) a gene cluster for alternative lipid biosynthesis (HMPREF1077_RS03620 to HMPREF1077_RS03635) in 5 mM cholate or 5 mM taurocholate; and (d) an NDP-sugar epimerase capD (BACUNI_RS12910) in 5 mM taurocholate. All inserts include 200 bp upstream of the gene(s).

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