Fig. 2: Deuterium loss from D5-glutamate is extraction water dose dependent.
From: A diverse proteome is present and enzymatically active in metabolite extracts
a Abundance of D5-glutamate across extraction conditions, from AMW20 to AMW60. D5-glutamate was added at resuspension (mean ± SD, n = 3 technical replicates per group). b Abundance of unlabeled, sample-originating glutamate across extraction conditions, from AMW20 to AMW60. (mean ± SD, n = 3 technical replicates per group). c Abundance of D4-glutamate across extraction conditions, from AMW20 to AMW60. D5-glutamate was added at resuspension (mean ± SD, n = 3 technical replicates per group). d D8-tryptophan is unaffected by extraction water content. D8-tryptophan was added at resuspension (mean ± SD, n = 3 technical replicates per group).
