Fig. 6: Directed evolution of GkOYE for MBH reaction. | Nature Communications

Fig. 6: Directed evolution of GkOYE for MBH reaction.

From: Unlocking the function promiscuity of old yellow enzyme to catalyze asymmetric Morita-Baylis-Hillman reaction

Fig. 6

a The results of Trp scanning. b The results of Y169 sites saturation mutation. c Evolved final variants GkOYE.11 and GkOYE.13 as orthogonal biocatalysts for enantioselective MBH reaction. d Yield and e.r. values of representative mutants during directed evolution for producing (R)-3. The reactions were carried out using 5 mM 1, 1 mM 2, and 100 μM purified protein in PBS (pH 10.0) with 3% methanol (MeOH) as a co-solvent. e Yield and e.r. values of representative mutants during directed evolution for producing (S)-3. n = 3 independent biological experiments. Data are presented as mean values ± SD. Source data are provided as a Source Data file.

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