Table 2 Kinetic parameters of different mutants

From: Unlocking the function promiscuity of old yellow enzyme to catalyze asymmetric Morita-Baylis-Hillman reaction

Enzyme

Km (1) [mM]

kcat (1) [h−1]

kcat/Km (1) [mM−1h−1]

Km (2) [mM]

kcat (2) [h−1]

kcat/Km (2) [mM−1h−1]

GkOYE.8

4.28

0.64

0.15

15.98

1.65

0.10

GkOYE.9

2.00

0.65

0.33

12.44

1.59

0.13

GkOYE.11

1.70

0.7

0.41

11.22

1.72

0.15

GkOYE.12

4.50

0.60

0.13

17.28

1.52

0.09

GkOYE.13

1.91

0.68

0.36

12.10

1.66

0.14

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