Fig. 2: BMP4 diverts cell fate toward extra-embryonic lineages.
From: Cell fate decision by a morphogen-transcription factor-chromatin modifier axis
a Flow chart shows the single-cell RNA sequencing under BMP4+ and BMP4− conditions after 7 days induction. b and c UMAP projection of 30,769 cells from MEF, ESC, and JGES reprogramming at day 7, colored by conditions or cell types, MEF and ESC data are from other’s work35. d Heatmap shows differentially expressed genes (DEGs) of four cell types (two-sided t-test, p-value < 0.01 and log fold change > 0.2). e Bar plots showing the representative Gene Ontology (GO) terms. One-sided Fisher’s exact test was used to perform enrichment, and terms with Benjamini–Hochberg adjusted p-value < 0.1 were selected. The enrichment was performed using the R package clusterProfiler36. f Stack bar plots showing the proportion of cell types between ±BMP4 conditions. g Dot plot showing the mean and percentage (expression = 0, pct) of PrECLCs marker genes between PrECLCs and the other cells at day 7. h Bar plot for Oct4 GFP positive iPS colonies numbers of indicated gene expression through JGES reprogramming, data are mean ± s.d., two-sided, unpaired t-test; n = 3 independent experiments, error bars here represent mean with SD. i Immunofluorescence staining shows the PrECLCs shape clone in BMP4+ condition at day 9 are GATA4 and LAMA1 dual positive, scale bar = 250 μm, n = 3 independent experiments.
