Fig. 3: BMP4 targets Sall4 for specifying alternative fate.
From: Cell fate decision by a morphogen-transcription factor-chromatin modifier axis
a Histogram shows Oct4 GFP positive iPS colonies numbers of Oct4 plus Sox2 reprograming with or without BMP4 treatment, data are mean ± s.d., two-sided, unpaired t-test; n = 3 independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001. b Schema chart shows the single-factor dropout strategy of JGES. c Histogram shows Oct4 GFP positive iPS colonies numbers under different factor dropout in JGES reprogramming at day 7 with or without BMP4 treatment, data are mean ± s.d., two-sided, unpaired t-test; n = 3 independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001. d Histogram shows Oct4 GFP positive iPS colonies numbers of JGES reprogramming plus Oct4 with or without BMP4 treatment, data are mean ± s.d., two-sided, unpaired t-test; n = 3 independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001. e Histogram shows Oct4 GFP positive iPS colonies numbers under single factor dropout in JGESO reprogramming at day 7 with or without BMP4 treatment, data are mean ± s.d., two-sided, unpaired t-test; n = 3 independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001. f Histogram shows Oct4 GFP positive iPS colonies numbers of OS plus JGE reprograming system with or without BMP4 treatment, data are mean ± s.d., two-sided, unpaired t-test; n = 3 independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001. g Histogram for Oct4 GFP positive iPS colonies numbers of OS plus DsRed,  Jdp2, Esrrb, Glis1, Sall4 experiments under BMP4+ and BMP4− conditions at Day 7, data are mean ± s.d., two-sided, unpaired t-test; n = 3 independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001. h Histogram for Oct4 GFP positive iPS colonies numbers of Glis1, Jdp2, Esrrb, JGE in OS plus Sall4 reprogramming system under BMP4+ and BMP4− conditions at Day 7, data are mean ± s.d., two-sided, unpaired t-test; n = 3 independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001. i and j Histograms show the qPCR results of PrE gene relative expression level of every group, n = 3 independent experiments, error bars here represent mean with SD.
