Fig. 2: Sub-tomogram average of RSV M.

a Model points for the center of sub-tomograms used in sub-tomogram averaging of RSV M are shown as squares and positioned above a tomographic slice from the corresponding virion segment (binned by 6 IsoNet processed volume) (n = 33). b Single slice from a sub-tomogram average of RSV M oriented with the M lattice shown underneath the viral membrane. Dashed lines indicate the z-position of the slice in d. c Isosurface rendering of RSV M sub-tomogram average with model fitting of the M dimer (PDB: 4v23). Models are shown as ribbon diagrams with the individual monomers differentially colored (dark blue and cyan). d Single slice from sub-tomogram average from b. e Isosurface of RSV M as viewed from the membrane toward the viral interior as in (d) with models of the M-dimer fit in to better visualize lattice organization. f Isosurface of M layer with model fitting of the M-dimer viewed from viral interior overlaid with the average center-to-center distances between individually fit dimers as well as the angles between dimers. Source data are provided as a Source Data file. Red arrows point to examples of map density between dimers that is not occupied by fitting 4v23. Density map in (b–f) is an average from n = 38,771 sub-tomograms. 50 nm scale bar for a, 5 nm scale bar for b–f.