Fig. 5: B3galt5 whole-body knockout exacerbated HFD-induced systemic inflammation in mice. | Nature Communications

Fig. 5: B3galt5 whole-body knockout exacerbated HFD-induced systemic inflammation in mice.

From: B3galt5 functions as a PXR target gene and regulates obesity and insulin resistance by maintaining intestinal integrity

Fig. 5

a The mRNA expression of inflammation-related gene Il-1β, Il-6, Mcp-1 and Tnf-α in liver (n = 8 for WT, n = 6 for KO). b The protein levels of IL-1β, IL-6, Mcp-1 and Tnf-α in liver determined by Elisa (n = 12 for WT, n = 10 for KO). c The mRNA expression of inflammation-related gene Il-1β, Il-6, Mcp-1 and Tnf-α in adipose tissue (n = 7 per group). (d) The protein levels of IL-1β, IL-6, Mcp-1 and Tnf-α in adipose tissue determined by Elisa (n = 7 per group). e, f Immunofluorescence staining of F4/80 in the liver (e) and adipose tissue (f). Scale bar: 50 μm. g FACS analysis of M1 and M2 phenotype of macrophages in eWAT of WT and B3galt5 KO mice fed HFD for 12 weeks (n = 4 per group). WT wild type, KO B3galt5 whole-body knockout, M1 type 1 macrophages, M2 type 2 macrophages, eWAT epididymal white adipose tissue. Data are mean ± SEM. Significance was determined using unpaired two-tailed Student’s t test. Source data are provided as a Source Data file.

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