Fig. 7: Intestinal specific B3galt5 knockout accelerates HFD-induced obesity and systemic inflammation. | Nature Communications

Fig. 7: Intestinal specific B3galt5 knockout accelerates HFD-induced obesity and systemic inflammation.

From: B3galt5 functions as a PXR target gene and regulates obesity and insulin resistance by maintaining intestinal integrity

Fig. 7

B3galt5f/f and B3galt5△IEC mice were fed with HFD for 12 weeks. a, b Appearance (a) and growth curve (b) of B3galt5f/f and B3galt5△IEC mice (n = 7 for B3galt5f/f, n = 6 for B3galt5△IEC). c, d Representative photographs of eWAT (c), iWAT (d). (e) H&E staining of eWAT and iWAT. Scale bar: 50 μm. f–g Blood glucose concentrations during GTT (1 g/kg; f) and ITT (1.5 U/kg; g) in B3galt5f/f and B3galt5△IEC mice (n = 7 for B3galt5f/f, n = 6 for B3galt5△IEC). h Serum insulin and leptin levels in B3galt5f/f and B3galt5△IEC mice (n = 7 for B3galt5f/f, n = 6 for B3galt5△IEC). i The protein levels of IL-1β, IL-6, Mcp-1 and Tnf-α in liver determined by Elisa (n = 6 for B3galt5f/f, n = 10 for B3galt5△IEC). j Immunofluorescence staining of F4/80 in liver. Scale bar: 50 μm. k The protein levels of IL-1β, IL-6, Mcp-1 and Tnf-α in adipose tissue determined by Elisa (n = 6 for B3galt5f/f, n = 10 for B3galt5△IEC). l Immunofluorescence staining of F4/80 in adipose tissue. Scale bar: 50 μm. m Portal vein serum endotoxin level (n = 7 for B3galt5f/f, n  = 6 for B3galt5△IEC). n The concentration of FITC-dextran in serum of B3galt5f/f and B3galt5△IEC mice fed with HFD for 12 weeks (n = 5 per group). o Aician blue staining and immunofluorescence staining of Muc2 in colon. Quantification of mucus layer thickness (right; n = 14 for B3galt5f/f, n = 16 for B3galt5△IEC). Scale bar: 50 μm. B3galt5f/f B3galt5 floxed mice, B3galt5△IEC intestine-specific B3galt5-deficient mice, eWAT epididymal white adipose tissue, iWAT inguinal white adipose tissue, GTT glucose tolerance test, ITT insulin tolerance test. Data are mean ± SEM. All the data were assessed form normal distribution before statistical analysis. Significance was analyzed using two-way analysis of variance (ANOVA) with Sidak’s multiple comparisons test (Fig. 7b, f and g). The remaining statistical differences were determined using unpaired two-tailed Student’s t test. Source data are provided as a Source Data file.

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