Fig. 1: Fluoxetine increases the proliferation of skeletal muscle stem cells, the functional vascularization, and the muscle strength of the skeletal muscle.
From: Serotonin reuptake inhibitors improve muscle stem cell function and muscle regeneration in male mice
a Schematic representation of fluoxetine (FLX) delivery. b Representative immunostaining TA sections of control and FLX-treated mice; sections display GFP (SCs marker, green), Laminin (extracellular matrix, white), Hoechst (nuclei, blue), the arrows indicate SCs. Scale bars indicate 50 µm. c Quantification of the SCs number by GFP immunostaining on TA sections from control and FLX-treated Tg:Pax7nGFP mice (n = 11 mice per condition from 2 independent experiments, p = 0.0014). d Representative immunostaining TA sections of control and FLX-treated Flk1GFP/+ mice; sections display GFP (endothelial cells marker, green). Scale bars indicate 50 µm. e Quantification of the vessels number by GFP immunostaining on TA sections from control and FLX-treated Flk1GFP/+ mice (n = 5 mice per condition, p = 0.0079). f Representative immunostaining Matrigel® plugs sections of control and FLX-treated C57Bl6 mice; sections display CD31+ cells (endothelial cell marker, red), Desmin (myoblasts marker, green), Hoechst (nuclei, blue). Scale bars indicate 50 µm. g Quantification of the vessels number by CD31 immunostaining on cross sections of Matrigel® plugs from control and FLX-treated recipient mice (n = 6 mice per condition, p = 0.0022). h In vivo forelimb grip strength of control and FLX-treated C57Bl6 mice (n = 12 mice per condition from 2 independent experiments, p = 0.0068). i Maximal aerobic velocity (VO2 max in m/min) determined by treadmill exercise of FLX-treated and control C57Bl6 mice (n = 10 mice per condition from 2 independent experiments, p = 0.0391). j Quantification of percent fiber typing by MyHC subtypes immunostaining on TA sections from control and FLX-treated C57Bl6 mice (n = 5 mice per condition, p = 0.0079, p = 0.0159, p = 0.0317, p = 0.0079, respectively). All values are represented as median with interquartile range. All data analyses were performed with the two-tailed Mann–Whitney test. *p ≤ 0.05, **p ≤ 0.01. Source data are provided as a Source Data file.
