Fig. 7: Excessive activity of BRD9/STAT1 axis contributes to the compromised OC differentiation process after loss of Arid1a.
From: ARID1A safeguards the canalization of the cell fate decision during osteoclastogenesis
a The protein expression of ARID1A and BRD9 in BMCs from 4-week-old male LysM-Cre;Arid1afl/fl mice and littermate control mice after RANKL-induction, as measured by western blot. b GSEA analysis of DEG profiles in bulk RNA-seq between the control group and LysM-Cre;Arid1afl/f group. c The mRNA expression of Stat1 and Ifnb1 in BMCs from 4-week-old male LysM-Cre;Arid1afl/fl mice and littermate control mice after RANKL-induction, as measured by qPCR. n = 3 biologically independent samples. d TRAP staining and quantification analysis of iBRD9 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1afl/fl mice and littermate control mice after RANKL-induction. Scale bar, 100 μm. n = 5 biologically independent samples. e The mRNA expression of Mmp9, Acp5 and Dcstamp and in iBRD9 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1afl/fl mice and littermate control mice after RANKL-induction. n = 4 biologically independent samples for Mmp9 and Acp5. n = 3 biologically independent samples in Dcstamp. f The protein expression of ARID1A, MMP9, and CTSK in iBRD9 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1afl/fl mice and littermate control mice after RANKL-induction, as measured by western blot. All data in this figure are represented as mean ± SD. Two-tailed Student’s t-test for c. One-way ANOVA with Tukey’s multiple comparisons test for d and e. All experiments were performed in triplicates unless otherwise stated. Source data and exact p values are provided in the Source data file.
