Fig. 4: The 44 aa N-terminal domain is required for FlhF function in Shewanella.

a Microscopic images of the indicated S. putrefaciens strains expressing FlhF-mVenus. The top row shows the phase contrast images; the row below shows the corresponding fluorescence images. The panel at the bottom displays an enlargement of the boxed areas within the fluorescence images. Fluorescent FlhF-mVenus foci are marked with a white arrow. The scale bar equals 3 μm. b Quantification of the FlhF-mVenus localization patterns in S. putrefaciens based on the microscopy images shown in a. Data was obtained from three individual experiments (n = 3) where at least 327 cells were counted for each biological replicate of each strain (****p < 0.0001, two-way ANOVA, ns = not significant; here p = 0.9663). Data are presented as mean values together with the corresponding ± standard error of mean (SEM). c Microscopic images of hook stains from the indicated S. putrefaciens strains with Alexa Fluor 488-C5-maleimide dye. The top row shows the phase contrast images, while the lower row shows the corresponding fluorescence images. Fluorescent hooks are marked with a white arrow. The scale bar equals 3 μm. d Quantification of the hook localization pattern in S. putrefaciens based on the microscopy images shown in Fig 4c. Data was obtained from three individual experiments (n = 3) where at least 330 cells were counted for each biological replicate of each strain (****p < 0.0001, two-way ANOVA). Data are presented as mean values together with the corresponding ± standard error of mean (SEM). Source data are provided in the Source Data file.