Fig. 5: The Shewanella HubP-FlhF-FliG recruitment cascade can be rebuilt in Escherichia coli.
From: Polar confinement of a macromolecular machine by an SRP-type GTPase
a Microscopic images of the indicated E. coli DH5α strains containing the expression plasmids for the wild-type version of HubP (SpHubP) or fluorescently tagged versions of S. putrefaciens HubP (SpHubP), FlhF (SpFlhF) or FliG (SpFliG). The left row shows the phase contrast images, while the right row shows the corresponding fluorescence images. Fluorescent foci of the respective fluorescently labeled protein are marked with a white arrow. The scale bar equals 3 μm. b, c Quantification of the localization pattern of SpFlhF (b) or SpFliG (c) with SpFlhF in E. coli DH5α based on the previous microscopy images. Data was obtained from three individual experiments (n = 3) where at least 310 cells were counted for each biological replicate of each strain (****p < 0.0001, ***p = 0.0001, **p = 0.0011, *p = 0.014; two-way ANOVA). Data are presented as mean values together with the corresponding ± standard error of mean (SEM). Source data are provided in the Source Data file.
