Fig. 4: Archaellins in N-glycosylation mutants display reduced densities adjacent to modified asparagine residues.

Cryo-EM maps (shown as meshes) and models of the close vicinities of the three N-glycosylation sites in Hbt. salinarum archaellins from the parent (WT) (magenta), Δagl27 (blue), and Δagl26 (green) strains. Glycans modeled at positions N80 and N93 were removed to allow a clear view of the density reductions adjacent to these residues in archaellins from the N-glycosylation mutant strains.