Fig. 2: Higher concentration of 7β,27-DHC treatment increase open probability of sPC-1/PC-2 inward currents.
From: Cilia-enriched oxysterol 7β,27-DHC is required for polycystin ion channel activation
A Representative inside-out single channel recordings of cells overexpressing sPC-1/2 with intracellular application of 1 μM to 50 μM 7β,27-DHC. Red dotted lines indicate closed (C) and open (O) states of a single channel. B Single-channel conductance after intracellular application of 1 μM to 50 μM 7β,27-DHC. green: 1 μM (n = 3), blue: 2.5 μM (n = 3), black: 5 μM (n = 4) and orange: 50 μM (n = 4) C Normalized absolute open probability of sPC-1/2 obtained from −100 mV to +100 mV during 10 s of recording. The absolute open probability was normalized to the maximum open probability. 1 μM (n = 3), 2.5 μM (n = 3), 5 μM (n = 4) 50 μM (n = 4). Two-tailed unpaired Student’s t-test, *P < 0.05. Error bars: mean ± SEM.
