Fig. 5: 7β,27-DHC potentiates ciliary polycystin-mediated currents.
From: Cilia-enriched oxysterol 7β,27-DHC is required for polycystin ion channel activation
A Representative excised inside-out single channel recordings of primary cilia in IMCD-3 cell without (left) and with 5 μM 7β,27-DHC (right). Single channel recordings were obtained between +100 mV and −100 mV at 20 mV increments. Red dotted line indicates the close state of the channel. B Representative image of ARL13B-GFP expressing IMCD-3 cells. Primary cilia (green) form a giga-ohm seal with glass electrode (right). C Normalized absolute open probability of ciliary channels. Popen of ciliary channels from IMCD-3 cells without (n = 4) and with 5 μM 7β,27-DHC (n = 4) D Single-channel conductance of ciliary channel recordings shown in 3 Å. Black and red squares indicate the averaged current amplitudes of ciliary channels from IMCD-3 cells without (n = 4) or with 5 μM 7β,27-DHC (n = 4) application, respectively. Dotted line indicates the fitting to the linear equation. Bar graph insert shows comparison of ciliary channel conductance without or with 5 μM 7β,27-DHC. Error bars: mean ± SEM.
