Fig. 2: Assembly and characterization of switchback DNA.
From: The unusual structural properties and potential biological relevance of switchback DNA
a Non-denaturing polyacrylamide gel electrophoresis (PAGE) analysis of homodimer switchback DNA. b Non-denaturing PAGE analysis of heterodimer switchback DNA. c Fluorescence experiments showing the formation of switchback DNA with strands in parallel orientation. d Percent fluorescence reduction during switchback DNA formation. e CD spectra of conventional duplex and heterodimeric switchback DNA. f UV thermal melting temperatures (Tm) of conventional duplex and switchback DNA. g Melting temperature of heterodimer switchback DNA at different Mg2+ concentrations. h Isothermal titration calorimetry (ITC) thermograms of conventional duplex and heterodimer switchback DNA. i Thermodynamic parameters of conventional duplex and switchback DNA. j Scheme of switchback DNA and conventional duplex with 1 or 2 mismatches. k Non-denaturing PAGE analysis of structures with mismatches. l, m Melting temperatures and difference in melting temperature compared to perfectly matched sequences for conventional duplexes and switchback DNA. Data represent mean and error propagated from standard deviations of experiments performed in triplicates (n = 3 independent experiments). The data shown in (a), (b) and (k) are representative of experiments performed multiple times (n > 3) with similar reproducible results. Source data are provided as a Source Data file.
