Fig. 4: Nb14, 22, 77 and 84 inhibit RAS signaling through the Raf/MEK/ERK pathway as assessed by ERK1/2T202/Y204 phosphorylation levels.
From: Allosteric nanobodies to study the interactions between SOS1 and RAS
a, b HEK293T cells expressing GFP or the indicated Nbs were serum-starved, stimulated with 10% serum, and analyzed by WES capillary immunoassay. c, d ERK1/2 phosphorylation. Values are means of phosphorylated (p) relative to non-phosphorylated protein levels ± SEM for (a, b), respectively. The experiments were repeated independently three times. Source data are provided as a Source Data file.
