Fig. 1: Schematics of in situ RNA C-to-U editing mediated by recombinant APOBEC1-Nanobody (APO1-Nb). | Nature Communications

Fig. 1: Schematics of in situ RNA C-to-U editing mediated by recombinant APOBEC1-Nanobody (APO1-Nb).

From: High-sensitivity in situ capture of endogenous RNA-protein interactions in fixed cells and primary tissues

Fig. 1

a Experimental procedure of INSCRIBE. After cell or tissue fixation, the RBP-targeting antibody (Ab) and the APO1-Nb are incubated sequentially, followed by RNA editing in situ at 37 °C overnight. RNAs are harvested for RNA-seq library. Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license. b Workflow describing data analysis for INSCRIBE. The RNA sequences are aligned to the transcriptome, followed by C-to-U edit quantification by SAILOR and edit clusters identification by FLARE. Subsequently, confident edit clusters are determined by intersecting clusters from three replicates and subtracting the Buffer-only control clusters. UTR: untranslated region.

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