Fig. 3: RNF213 is essential for Treg cells to suppress T cell-mediated EAE.

A Schematic of experimental design of adoptive CD4⁺ T cell transfer assays. In detail, Rag1^−/− mice were transferred with 1 × 10⁶ CD44⁻ CD62L⁺ CD4⁺ T cells (Tn) from CD45.1⁺ mice alone (n = 5) or in combination with Tn from CD45.2⁺ WT or Rnf213^−/− Tregs. B The graph shows the clinical score of EAE (n = 5 per group). C Concentration of IL-17 and IL-10 in serum was measured by ELISA on day 20. D The cells infiltrating the central nervous system (CNS) were harvested, and the percentages of CD45.1⁺ and CD45.2⁺ in the CD4⁺ T cell populations was determined. Pooled data of the percentages and cell counts are presented in the right panel. E, F Flow cytometry of IFNγ⁺, IL-17A⁺ CD4⁺ cells (E) or CD4⁺ CD25⁺ Foxp3⁺ Treg cells (F) in CNS of recipient mice on days 20 during EAE. Pooled data of the percentages and cell counts are presented in the right panel. Data shown are the mean ± SD. Ns, no significance, *P < 0.05, **P < 0.01, and ***P < 0.001. P values were calculated using a two-sided, unpaired Student’s t-test in (B–F). N = 5 (C–F) repeats from three independent experiments. Source data are provided as a Source Data file.