Fig. 3: Rapid nascent transcriptional changes upon Firre induction.

a Schematic of PRO-seq experimental setup. b Genome browser tracks of bidirectional transcription of genes that were (Shf, Duox1) and were not (Ep300) differentially expressed upon Firre induction. Red and gray areas indicate reads on the plus and minus strand, respectively. Arrows denote the bidirectional center. c Metaplot of PRO-seq signal at the differentially expressed sites of bidirectional transcription (padj < 0.05, n = 550, Wald test) around the bidirectional center (μ) at the 0-, 15-, and 30-min time points. d Genome browser tracks showing the regulatory events at the Apod locus upon Firre induction. The arrow indicates an internal TSS. e Volcano plot of the log₂ fold changes of nascent transcription over the gene body at 30 vs 0 min. Genes with padj <0.05 (Wald test) are indicated in red.