Table 1 Fluorescent scFv immuno-probes generated in this work

From: Multiplexed volumetric CLEM enabled by scFvs provides insights into the cytology of cerebellar cortex

Target

mAb clone no.

Linker length

Fluorescent dyes conjugated

Link of expression plasmid at Addgene

GFP

N86/38

3×

5-TAMRA

https://www.addgene.org/204419/

Calbindin

L109/57

4×

5-TAMRA, Alexa Fluor 488, Alexa 594

https://www.addgene.org/204421/

GFAP

N206B/9

3×

5-TAMRA, Alexa 488

https://www.addgene.org/204420/

VGluT1

N28/9

4×

5-TAMRA, Alexa Fluor 532

https://www.addgene.org/204424/

PSD-95

K28/43

4×

5-TAMRA

https://www.addgene.org/204422/

Kv1.2

K14/16

4×

Alexa Fluor 594

https://www.addgene.org/204425/

Parvalbumin

L114/81

4×

5-TAMRA, Alexa Fluor 647

https://www.addgene.org/204423/

Neuropeptide Y

L115/13

4×

Alexa Fluor 594

https://www.addgene.org/204426/

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