Fig. 1: Interneuron heterogeneity of the human striatum determined by single-nucleus RNA-sequencing.

A Schematic overview of the experimental design. B Number of interneuron nuclei sequenced in the present study (labeled as AMM) vs. two other previous works. * Note that the only interneuron nuclei shown from Lee et al.’s study correspond to its control donors. C Number of human samples included in this study (AMM) vs. two previous works. * Note that the only samples shown from Lee et al.’s study correspond to its control donors. D Uniform manifold approximation projection (UMAP) of the snRNA-seq data of the nuclei labeled as interneurons, colored by interneuron subclass. The corresponding main class is indicated on the right. E Normalized expression of classical interneuron marker genes by each of the 14 interneuron subclasses identified. The expression corresponding to each interneuron subclass is colored following the same color scheme as in panel (D). F Heatmap showing the expression of selected marker genes for each of the 14 interneuron subclasses identified. The expression of each gene is normalized by its maximum value across all nuclei. The dendrogram above the heatmap indicates the proximity across subclasses based on the average Pearson correlation coefficient across all genes between each pair of subclasses. CN caudate nucleus, IN interneuron, Pu putamen.