Fig. 3: DHX9 SUMOylation prevents the accumulation of DNA damage.
From: DHX9 SUMOylation is required for the suppression of R-loop-associated genome instability
a–e HeLa derivative clones transfected with negative control or DHX9 siRNAs for 1 d were induced to express SFB-DHX9WT (WT-2) or SFB-DHX9K120R (K120R-13) by doxycycline for 3 d, followed by treatment with DMSO or CPT (1 μM) for 1 h. The percentage and intensity of γH2AX (red) and FLAG (green) staining were determined. a Representative images of three independent experiments. Scale bar = 5 μm. b The percentage of γH2AX foci-positive cells was quantified. Data are presented as mean ± SD with dots indicating results from three independent experiments (ns = no significance) analyzed by one-way ANOVA. c Intensity of γH2AX foci was quantified in DMSO and CPT-treated HeLa clones. Representative data of three biological replicates analyzed by two-sided t-test. Cells with background staining were colored black. The black line indicates the median. d, e The correlation between SFB-DHX9 expression and γH2AX foci intensity in WT and mutant clone cells treated with DMSO were plotted in 2D, respectively. f Representative Western blot of three independent experiments shows the levels of endogenous and SFB-tagged DHX9 in different HeLa derivative lines. Source data are provided as a Source Data file.
