Fig. 2: EGCs display an activated and immunomodulatory phenotype in CRC.

Transcriptome analysis of in vitro primary embryonic neurosphere-derived EGCs alone or stimulated with healthy conditioned medium (H-CM) or tumor microenvironment conditioned medium (TME-CM) at different time points (6 h, 12 h, and 24 h, n = 4). a Schematic representation of the in vitro tumor EGCs model. b Principal component analysis (PCA) plot of EGCs gene signature identified by 3´mRNA bulk RNA-seq. Each dot represents an individual sample. c Heatmap showing the transcriptional modules identified by weighted gene correlation network analysis (WGCNA). d Heatmap of differentially expressed genes in modules 4, 7, and 8 of in vitro murine EGCs stimulated for 24 h with H- or TME-CM. e Gene set enrichment analysis for the differentially up- and down-regulated genes in TME-CM versus H-CM stimulated EGCs after 24 h (n = 4). Source data are provided as a Source Data file.