Fig. 8: IL-1R induced-CRC EGC phenotype in patients with CRC.

a Representative image showing S100 (magenta), CD68 (green) and Pancytokeratin (PANCK, white) in a human CRC tissue section (scale bar 200 µm and 50 µm). Image representative of ten stainings. b, c TCGA COAD and READ patients stratified based on their expression of the EGCs signature genes (n = 309 EGCs low, n = 67 EGCs high). Kaplan-Meier overall survival curve for EGCs high and low patients (b). The proportion of EGCs high and low patients classified based on high or low SPP1⁺ TAMs gene signature expression (c). d, e Transcriptome analysis of tumor EGCs in CRC patients (KUL3 Dataset³³, n = 5). Volcano plot of differentially expressed genes between healthy and tumor EGCs (d), highlighting genes defining the tumor EGCs signature. Gene Set Enrichment Analysis presenting GO terms of interest (e). f Violin plot showing expression of IL1B in the tumor-infiltrating myeloid cell clusters of human CRC in the KUL3 Dataset³³ (n = 5). Statistical analysis: Mantel Cox test (b) and Wilcoxon test with Bonferroni correction using Seurat (d). Source data are provided as a Source Data file.