Fig. 3: The abundance of triglycerides is lower following GSH depletion in mice.
From: Glutathione synthesis in the mouse liver supports lipid abundance through NRF2 repression
Lipidomic analysis of (A) adipose tissue, (B) liver tissue, and (C) serum in KO (n = 4) compared to WT (n = 4) mice. Black data points = lipid species with a p < 0.05 and log2 fold change >1. Red data points = triglycerides. D Triglyceride levels in the serum of WT (n = 4) and KO (n = 4) mice. An unpaired two-tailed t-test was used to determine statistical significance (WT vs. KO P value = 0.0119). E Triglyceride levels in the serum for WT (n = 4) and KO (n = 4) mice fed with either normal chow or a high-fat diet (HFD) for 14 days post-treatment with tamoxifen. A one-way ANOVA with subsequent Tukey’s multiple comparisons test was used to determine statistical significance (WT Normal Chow vs. KO Normal Chow P value = 0.0307, WT Normal Chow vs. KO HFD P value = 0.9683, KO Normal Chow vs. KO HFD P value = 0.0447). F Percent change in body weight of WT (n = 7) and KO mice fed with either normal chow (n = 7) or a high-fat diet (HFD; n = 9) 14 days post-treatment with tamoxifen. A one-way ANOVA with subsequent Tukey’s multiple comparisons test was used to determine statistical significance (WT Normal Chow vs. KO Normal Chow P value < 0.0001, WT Normal Chow vs. KO HFD P value =<0.0001, KO Normal Chow vs. KO HFD P value = 0.0006). G Relative abundance of select SCD1 fatty acids products in the liver from WT (n = 4) and KO (n = 4) mice. 16:0 = palmitic acid; 16:1;2 O= oxidized palmitoleic acid; 18:0 = stearic acid; 18:1 = oleic acid. A two-way ANOVA with subsequent Šidák’s multiple comparisons test was used to determine statistical significance (WT vs. KO: 16:0 P value = 0.0603, 16:1;2 O P value = 0.9991, 18:0 P value = 0.0377, 18:1 P value = 0.9268). H Relative abundance of select triglyceride species in the serum from WT (n = 4) and KO (n = 4) mice. A two-way ANOVA with subsequent Šidák’s multiple comparisons test was used to determine statistical significance (WT vs. KO for all comparisons indicated: **** P value < 0.0001). Indicated n values represent biologically independent samples from mice. Data are shown as mean ± SEM. ns not significant, * P value < 0.05, ** P value < 0.01, **** P value < 0.0001.
