Fig. 9: SKF96365 blocks [Ca²⁺]_ex drop and alleviates IMQ-induced psoriasis.

a–c 10-week-old mice were treated with 62.5 mg cream containing 5% IMQ onto abdominal skin daily for 2 days (n = 6 mice). DMSO and 100 µM SKF96365-pretreated T cells were transferred into mice via tail vein injection. The transferred T cells were labeled with Cell Tracer 647. a Schematic diagram of IMQ-induced psoriasis mouse model. b Representative intravital micrographs of the transferred T cells arrested in skin postcapillary venules at psoriasiform lesions. Vessels were visualized by dextran Texas Red and CD31-Alexa Fluor 594 (red). Images are from Supplementary Movie 3. Time is shown in min:s. c Quantification of the arrested T cells in skin postcapillary venules. Data are presented as box-and-whisker plots showing the median (central line), 25th–75th percentile (bounds of the box), and 5th–95th percentile (whiskers). Each dot represents an individual movie. d–h 10-week-old R26-LSL-CEPIAexternal;Itgal-LSL-Clover;Itgb2-LSL-mRuby2;CD4-Cre mice were treated with 18 mg cream containing 5% IMQ onto each ear and injected with 10 mg/kg SKF96365 or DMSO daily for 7 days (n = 6 mice). d Schematic diagram of IMQ-induced psoriasis and SKF96365 treatment. e Changes in ear thickness relative to day 0 of IMQ application. f Representative H&E staining of ear sections of DMSO or SKF96365 treated mice on day 7 after IMQ treatment (left). Scale bar: 50 μm. Quantification of epidermal thickness (right). An average of at least 3 measurements per sample was calculated. g Quantification of αLβ2⁺ T cells in the ear on day 7 after IMQ treatment. h Representative immunofluorescent images of ear skin of mice treated with DMSO or SKF96365 (left). Scale bar: 100 μm. Right: Quantification of αLβ2⁺ T cells in each field (right). An average of at least 3 measurements per sample was calculated. Data represent the mean ± SD. ns, not significant (unpaired two-tailed Student’s t test). Source data are provided as a Source Data file.